Get Antigen Processing: Methods and Protocols PDF

By Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild (auth.), Peter van Endert (eds.)

ISBN-10: 1627032177

ISBN-13: 9781627032179

Antigen processing is a organic strategy that prepares antigens for the presentation to important cells within the immune procedure known as T lymphocytes. In Antigen Processing: tools and Protocols, specialist researchers within the box supply a entire set of protocols for learning presentation of antigens produced within the commonplace processing pathways for MHC type I and sophistication II molecules. The chapters keep on with chronology of intracellular processing occasions, finishing with attractiveness of peptide-MHC complexes on the phone floor by means of T lymphocytes. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and key pointers on troubleshooting and warding off identified pitfalls.

Authoritative and sensible, Antigen Processing: equipment and Protocols is designed for newcomers and specialists drawn to learning antigen processing.

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Of heat shock protein 70 peptide complexes from dendritic cell-tumor fusion cells. J Immunol 177(9):5946–5955 8. Kloetzel PM, Ossendorp F (2004) Proteasome and peptidase function in MHC-class-I-mediated antigen presentation. Curr Opin Immunol 16(1):76–81. doi:S095279150300178X (pii) 9. Asemissen AM, Keilholz U, Tenzer S, Muller M, Walter S, Stevanovic S, Schild H, Letsch A, Thiel E, Rammensee HG, Scheibenbogen C (2006) Identification of a highly immunogenic HLA-A*01-binding T cell epitope of WT1.

Voges D, Zwickl P, Baumeister W (1999) The 26S proteasome: a molecular machine designed for controlled proteolysis. Annu Rev Biochem 68:1015–1068 3. 4 A resolution. Nature 386:463–471 4. Groll M, Bajorek M, Kohler A, Moroder L, Rubin DM, Huber R, Glickman MH, Finley D (2000) A gated channel into the proteasome core particle. Nat Struct Biol 7:1062–1067 5. Sijts A, Sun Y, Janek K, Kral S, Paschen A, Schadendorf D, Kloetzel PM (2002) The role of the proteasome activator PA28 in MHC class I antigen processing.

12. Keep your cells on ice to prevent internalization of antibodies and protect your samples from light exposure. 13. You need an appropriate laser in your flow cytometer to excite your fluorescent dye and also detectors measuring the correct emission wavelength. 38 M. Basler and M. Groettrup 14. Do not count residual un-lysed erythrocytes. 15. It is useful to pulse female splenocytes with different concentrations of synthetic peptide (10−5 to 10−10 M) and use the pulsed cells as stimulators for hybridomas.

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Antigen Processing: Methods and Protocols by Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild (auth.), Peter van Endert (eds.)


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