By Curtis A. Williams, Merrill W. Chase
Tools in Immunology and Immunochemistry, quantity IV: Agglutination, supplement, Neutralization, and Inhibition presents details pertinent to direct and oblique agglutination reactions. This e-book covers numerous issues, together with complement-fixation techniques, isolation of supplement elements, hemolytic intermediates, complement-related proteins, and neutralization reactions.
Organized into 3 chapters, this quantity starts off with an summary of test-tube agglutinations which are most well-liked for blood grouping with saline agglutinins that require quite a lot of mins for agglutination. this article then describes blood crew antibodies that agglutinate pink blood cells suspended in saline. different chapters contemplate the classical pathway of supplement usage. This publication discusses to boot the complexity of occasions resulting in hemolysis of erythrocytes by way of supplement. the ultimate bankruptcy bargains with the facility of antitoxin to neutralize diphtheria toxin and explains the quantitative relationships among antigen and antibody.
This publication is a worthy source for immunologists, scientists, and study employees.
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Additional info for Agglutination, Complement, Neutralization, and Inhibition
Biol. 32, 453 (1968). " C. L . Finstad, G. W. Litman, J . Finstad, and R. A. Good, J. Immunol. 108, 1704 (1972). " C. L . Finstad, R. A. Good, and G. W. Litman, Ann. N. Y. Acad. Sei. 234, 170 (1974). " G. N . , J . W. Becker, B . A. Cunningham, G. R. Gunther, J . L . Wang, and G. M. Edelman, Ann. N. Y. Acad. Sei. 234, 369 (1974). *o G. W. G. Bird, Ann. N. Y. Acad. Sei. 234, 129 (1974). 6] A G G L U T I N A T I O N A N D FLOCCULATION TABLE II SELECTIVITY OF V A R I O U S L E C T I N S FOR H U M A N E R Y T H R O C Y T E S Specificity Anti-A Source Common name Phaseolus limensis Phaseolus lunatus Lima beans Sieva (small pole lima beans) Dolichos biflorus Horse gram Vicia cracca Vicia villosa Vicia pe,egrina Crotolaria aegyptica Crotolaria falcata Hyptos suaveolens Clitocyba nebularis Otala lactea Helix pomatia Anti-B Sophora japonica Bandeiraea simplicifolia Marasmius oreades Polyporus fomentarius Mushroom Snail (edible, im ported from N .
Lectin solutions are prepared by extraction of the appropriate part of the plant with physiological saline (extensive trials with hypo- and hyper tonic solutions of N a C l and other salts have not revealed any superior method of extraction). Seeds should be ground fine before extraction. F o r small amounts a mortar and pestle suffice; larger amounts can be ground in a suitable mill (an electric coffee mill serves well). The extraction m a y " W. C. Boyd, H. M. Bhatia, M . A. Diamond, and S.
00005%) tannic acid a n d incubated a t 3 7 ° for 15 minutes. 4 phosphatebuffered saline. One volume of the antigen solution of suitable concen tration (see T a b l e I I ) is then added to the cell suspension. T h e mixture is incubated for 30 minutes a t room temperature. 2 buffered saline, the cells are suspended in 1 volume of 1 % normal rabbit serum (the original " 2 . 5 % " concentra tion). J A t 4 ° these preparations m a y remain stable and suitably sensitive for hemagglutination for 2 to 3 days.
Agglutination, Complement, Neutralization, and Inhibition by Curtis A. Williams, Merrill W. Chase